USING MICROORGANISMS FOR SOIL PURIFICATION FROM HIGH-PARAFFIN CRUDE OIL

Microorganisms and Crude Oil

Crude oil is one of the most important energetic resources in the world. It is used as raw material in numerous industries, including the refinery-petrochemical industry, where crude oil is refined through various technological processes into consumer products such as gasoline, oils, paraffin oils, lubricants, asphalt, domestic fuel oil, vaseline, and polymers. Oil-derived products are also com...

Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms.

We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide solution. This method was used to construct large-insert soil metagenomic librar...

Screening of Microorganisms Isolated from Petroleum Oil Contaminated Soil for Vegetable Oil Conversion to Bio-diesel

Decreasing fossil fuel stocks has prompted research and development to find alternatives, particularly from renewable energy resources. Lower alcohol, fatty ester called as Bio-Diesel production using vegetable oils with the help of biological catalysts (Lipases) is being considered as one such good alternative. Soil samples in and around petroleum stock processing plant wereused to isolate fun...

Soil Microorganisms of High-Molecular-Weight DNA from Recovery, Purification, and Cloning

Marine biosurfactants, I. Screening for biosurfactants among crude oil degrading marine microorganisms from the North Sea.

Three bacterial strains of marine origin were isolated during a screening for biosurfactants among n-alkane degrading microorganisms. One strain-identified as Alcaligenes sp. MM1-produced a novel glucose lipid. In the case of Arthrobacter sp. EK 1 the well-known trehalose tetraester was found as major component. From another pure culture classified as Arthrobacter sp. SI 1, extracellular emulsi...